Abstract
Myocardin is a SAP (SAF-A/B, Acinus, PIAS) domain transcription factor that associates with serum response factor (SRF) to potently enhance SRF-dependent transcription. Here we describe two myocardin-related transcription factors (MRTFs), A and B, that also interact with SRF and stimulate its transcriptional activity. Whereas myocardin is expressed specifically in cardiac and smooth muscle cells, MRTF-A and -B are expressed in numerous embryonic and adult tissues. In SRF-deficient embryonic stem cells, myocardin and MRTFs are unable to activate SRF-dependent reporter genes, confirming their dependence on SRF. Myocardin and MRTFs comprise a previously uncharacterized family of SRF cofactors with the potential to modulate SRF target genes in a wide range of tissues.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites
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Blotting, Northern
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Cloning, Molecular
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DNA, Complementary
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Expressed Sequence Tags
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Gene Expression Regulation, Developmental*
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Glutathione Transferase / genetics
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Humans
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Mice
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Molecular Sequence Data
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Myocardin
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Myocardium / metabolism*
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Nuclear Proteins / genetics*
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Nuclear Proteins / metabolism*
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Recombinant Fusion Proteins / metabolism
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Sequence Alignment
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Sequence Homology, Amino Acid
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Serum Response Factor / genetics*
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Serum Response Factor / metabolism*
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Trans-Activators / genetics*
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Trans-Activators / metabolism*
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Transcription Factors
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Xenopus
Substances
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DNA, Complementary
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Glutathione Transferase
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Nuclear Proteins
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Recombinant Fusion Proteins
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Serum Response Factor
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Trans-Activators
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Transcription Factors
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Myocardin