CD4+ and CD8+ mediated cellular immune response to recombinant influenza nucleoprotein

Vaccine. 2002 Nov 1;20(31-32):3731-8. doi: 10.1016/s0264-410x(02)00355-9.

Abstract

The stimulatory properties of soluble recombinant influenza nucleoprotein (NP) on purified CD4(+) and CD8(+) T cells from young and elderly individuals were studied. Recombinant influenza NP failed to induce proliferation of resting CD4(+) and CD8(+) T cells in the absence of IL-2. Addition of small amounts of IL-2, however, led to strong proliferation of resting CD4(+) and CD8(+) T cells from young and elderly donors. NP-reactive CD4(+) and CD8(+) T cell lines from both age groups grew equally well under long-term culture conditions. T cell lines raised to live influenza virus could recognize recombinant influenza NP and showed a substantial proliferative response. Stimulation of CD8(+) T cells is presumably due to cross-presentation, as EBV-transformed MHC class I-positive cell lines, which are incapable of antigen processing, stimulated live influenza virus-reactive CD8(+) T cell lines when loaded with NP-derived immunodominant peptides but not following loading with the whole NP molecule. Vaccines containing recombinant influenza NP might confer cross-protective immunity and could therefore be especially useful in cases of major epidemics or pandemics.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Aging / immunology
  • Antigen-Presenting Cells / immunology
  • Antigen-Presenting Cells / metabolism
  • B-Lymphocytes / metabolism
  • B-Lymphocytes / virology
  • CD4 Antigens / immunology*
  • CD4-Positive T-Lymphocytes / immunology
  • CD4-Positive T-Lymphocytes / metabolism
  • CD8 Antigens / immunology*
  • CD8-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / metabolism
  • Cell Line, Transformed
  • Cell Separation
  • Cells, Cultured
  • Female
  • Herpesvirus 4, Human
  • Humans
  • Immunity, Cellular
  • Influenza A virus / immunology
  • Influenza Vaccines / immunology
  • Influenza Vaccines / metabolism
  • Interleukin-2 / pharmacology
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / immunology
  • Male
  • Nucleocapsid Proteins
  • Nucleoproteins / immunology*
  • Nucleoproteins / metabolism
  • RNA-Binding Proteins*
  • Recombinant Proteins / immunology*
  • Recombinant Proteins / metabolism
  • T-Lymphocyte Subsets / immunology
  • T-Lymphocyte Subsets / metabolism
  • Vaccines, Attenuated / immunology
  • Vaccines, Attenuated / metabolism
  • Vaccines, Synthetic / immunology
  • Vaccines, Synthetic / metabolism
  • Viral Core Proteins / immunology*
  • Viral Core Proteins / metabolism

Substances

  • CD4 Antigens
  • CD8 Antigens
  • Influenza Vaccines
  • Interleukin-2
  • NP protein, Influenza A virus
  • Nucleocapsid Proteins
  • Nucleoproteins
  • RNA-Binding Proteins
  • Recombinant Proteins
  • Vaccines, Attenuated
  • Vaccines, Synthetic
  • Viral Core Proteins