[Clinical significance of fluoroscopic patterns specific for the mitotic spindle in patients with rheumatic diseases]

Reumatismo. Jul-Sep 2002;54(3):232-7. doi: 10.4081/reumatismo.2002.232.
[Article in Italian]

Abstract

Objective: To determine the clinical significance of anti-NuMA and anti-HsEg5 antibodies in a group of patients affected with rheumatic diseases.

Materials and methods: Indirect immunofluorescence on HEp-2000 cells at serum dilution of 1:40 was used to examine 26 sera which had previously showed a "mitotic spindle" fluoroscopic pattern type during laboratory routine.

Results: 21 sera (80,7%) were identified with NuMA and 5 (19,3%) with HsEg5 patterns alone or associated with other ANA patterns. However only patients with isolated positivity and that is 15 with NuMA and 4 with HsEg5 stainings were included in this study. Of the NuMA positive patients 5 were affected with arthropathies associated to different forms of thyroiditis, 2 with seronegative arthritis, 2 with antiphospholipid syndrome, 1 with systemic lupus erythematosus (SLE), 1 with rheumatoid arthritis, 1 with sicca syndrome, 1 with undifferentiated connective tissue disease, 1 with Mycoplasma pneumoniae infection and 1 with retinal thrombosis. Of the HsEg5 positive patients 3 were affected with SLE and 1 with seronegative arthritis.

Conclusions: NuMA does not prevail in any defined rheumatic disease, while HsEg5 staining were more frequent (75%) in patients affected with SLE all of whom showing high antibody titres.

Publication types

  • Comparative Study

MeSH terms

  • Adenocarcinoma / pathology
  • Antibodies, Antinuclear / immunology
  • Antigens, Nuclear
  • Antiphospholipid Syndrome / blood
  • Antiphospholipid Syndrome / immunology
  • Arthritis / blood
  • Arthritis / immunology
  • Arthritis, Rheumatoid / blood
  • Arthritis, Rheumatoid / immunology
  • Autoantibodies / blood*
  • Autoantibodies / immunology
  • Autoimmune Diseases / blood
  • Autoimmune Diseases / immunology*
  • Cell Cycle
  • Cell Cycle Proteins
  • Centrosome / immunology*
  • Connective Tissue Diseases / blood
  • Connective Tissue Diseases / immunology
  • Fluorescent Antibody Technique, Indirect*
  • Fluorescent Dyes / analysis
  • Humans
  • Kinesin / analysis*
  • Kinesin / immunology
  • Laryngeal Neoplasms / pathology
  • Lupus Erythematosus, Systemic / blood
  • Lupus Erythematosus, Systemic / immunology
  • Microscopy, Fluorescence*
  • Nuclear Matrix-Associated Proteins
  • Nuclear Proteins / analysis*
  • Nuclear Proteins / immunology
  • Pneumonia, Mycoplasma / blood
  • Pneumonia, Mycoplasma / immunology
  • Sjogren's Syndrome / blood
  • Sjogren's Syndrome / immunology
  • Spindle Apparatus / immunology*
  • Thrombosis / blood
  • Thrombosis / immunology
  • Thyroiditis, Autoimmune / blood
  • Thyroiditis, Autoimmune / immunology
  • Tumor Cells, Cultured / immunology
  • Tumor Cells, Cultured / ultrastructure
  • Xenopus Proteins / analysis*
  • Xenopus Proteins / immunology

Substances

  • Antibodies, Antinuclear
  • Antigens, Nuclear
  • Autoantibodies
  • Cell Cycle Proteins
  • Fluorescent Dyes
  • KIF11 protein, Xenopus
  • KIF11 protein, human
  • NUMA1 protein, Xenopus
  • NUMA1 protein, human
  • Nuclear Matrix-Associated Proteins
  • Nuclear Proteins
  • Xenopus Proteins
  • Kinesin