A non-antibacterial oxazolidinone derivative that inhibits epithelial cell sheet migration

Chembiochem. 2002 Nov 4;3(11):1105-11. doi: 10.1002/1439-7633(20021104)3:11<1105::AID-CBIC1105>3.0.CO;2-S.

Abstract

We have developed a high-throughput assay for screening chemical libraries for compounds that affect cell sheet migration during wound closure in epithelial cell monolayers. By using this assay, we have discovered a new inhibitor of cell sheet migration. This compound (UIC-1005) is a 3,4-disubstituted oxazolidinone that bears an electrophilic alpha,beta-unsaturated N-acyl group required for activity. UIC-1005 also inhibits growth in an epithelial cell proliferation assay. The molecule does not display general toxicity at concentrations at which it potently inhibits cell sheet migration and growth. Unlike certain 3,5-disubstituted oxazolidinones, it exhibits no antibacterial activity. UIC-1005 therefore represents a new class of bioactive oxazolidinone derivative that may prove useful as a probe for signaling pathways leading to cell motility.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Cell Movement / drug effects
  • Dogs
  • Drug Evaluation, Preclinical / methods
  • Epithelial Cells / cytology
  • Epithelial Cells / drug effects*
  • Oxazolidinones / pharmacology*
  • Structure-Activity Relationship
  • Wound Healing / drug effects

Substances

  • Oxazolidinones