Role of the progesterone receptor (PR) in susceptibility of mouse mammary gland to 7,12-dimethylbenz[a]anthracene-induced hormone-independent preneoplastic lesions in vitro

Cancer Lett. 2002 Dec 15;188(1-2):47-52. doi: 10.1016/s0304-3835(02)00461-5.

Abstract

Glands of wild-type (WT) and progesterone receptor knockout (PRKO) mice were exposed to 7,12-dimethylbenz[a]anthracene (DMBA) while cultured in serum-free medium containing insulin, prolactin, aldosterone, and cortisol. Glands of WT but not PRKO mice responded to DMBA with epithelial hyperplasia after 10 days in this medium. After culture without prolactin and adrenocortical hormones for an additional 14 days, hyperplastic lesions were present only in glands of WT mice. We conclude that in the absence of PR, epithelial structures are resistant to the carcinogenic action of DMBA.

Publication types

  • Comparative Study

MeSH terms

  • 9,10-Dimethyl-1,2-benzanthracene / toxicity
  • Aldosterone / pharmacology
  • Animals
  • Breast / drug effects
  • Breast / pathology
  • Disease Susceptibility
  • Female
  • Hydrocortisone / pharmacology
  • Immunoenzyme Techniques
  • Insulin / pharmacology
  • Mammary Neoplasms, Experimental / etiology*
  • Mammary Neoplasms, Experimental / metabolism
  • Mammary Neoplasms, Experimental / pathology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Neoplasms, Hormone-Dependent / etiology*
  • Neoplasms, Hormone-Dependent / metabolism
  • Neoplasms, Hormone-Dependent / pathology
  • Organ Culture Techniques
  • Precancerous Conditions / etiology*
  • Precancerous Conditions / metabolism
  • Precancerous Conditions / pathology
  • Prolactin / pharmacology
  • Receptors, Progesterone / physiology*

Substances

  • Insulin
  • Receptors, Progesterone
  • Aldosterone
  • 9,10-Dimethyl-1,2-benzanthracene
  • Prolactin
  • Hydrocortisone