Abstract
Aza-peptide epoxides, a new class of irreversible protease inhibitors, are specific for the clan CD cysteine proteases. The inhibitors have second-order rate constants up to 10(5) M(-1) s(-1), with the most potent epoxides having the S,S stereochemistry. The aza-Asn derivatives are effective legumain inhibitors, while the aza-Asp epoxides were specific for caspases. The inhibitors have little or no inhibition with other proteases such as chymotrypsin, papain, or cathepsin B.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aza Compounds / chemical synthesis*
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Aza Compounds / chemistry
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Caspase Inhibitors
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Cathepsin B / antagonists & inhibitors
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Crystallography, X-Ray
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Cysteine Endopeptidases
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Cysteine Proteinase Inhibitors / chemical synthesis*
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Cysteine Proteinase Inhibitors / chemistry
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Epoxy Compounds / chemical synthesis*
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Epoxy Compounds / chemistry
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Kinetics
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Oligopeptides / chemical synthesis*
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Oligopeptides / chemistry
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Papain / antagonists & inhibitors
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Plant Proteins / antagonists & inhibitors
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Stereoisomerism
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Structure-Activity Relationship
Substances
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Aza Compounds
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Caspase Inhibitors
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Cysteine Proteinase Inhibitors
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Epoxy Compounds
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Oligopeptides
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Plant Proteins
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Cysteine Endopeptidases
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Cathepsin B
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Papain
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asparaginylendopeptidase