Characterization of the genomic and transcriptional structure of the CRX gene: substantial differences between human and mouse

Genomics. 2002 Nov;80(5):531-42. doi: 10.1006/geno.2002.6854.


We have previously shown that there is a temporal difference in human CRX: gene expression compared with that of mouse Crx. We have now characterized these genes at the genomic and transcriptional levels and here we expand on this earlier report. Human CRX: spans 25 kb and has six exons, and mouse CRX: spans 15 kb and has four exons. We isolated seven human and two mouse mRNAs generated by alternative splicing of a variable 5' untranslated region. The human and mouse genes share an evolutionarily conserved promoter, which contains OTX/CRX type and SP1/AP2 binding sites and drives expression of two conserved transcripts in both species. Additionally, the human gene has a second human-specific promoter, which has OTX/CRX type binding sites and drives expression of five other transcripts. Band shift assays have shown that six of the seven candidate OTX/CRX elements bind CRX in vitro, possibly implying that the gene can regulate its own expression. These data may account for the differences in temporal expression

In vivo: we have previously reported between these two species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions*
  • 5' Untranslated Regions
  • Alternative Splicing
  • Animals
  • Base Sequence
  • Binding Sites
  • Cell Line
  • Evolution, Molecular
  • Gene Expression Regulation, Developmental
  • Homeodomain Proteins / genetics*
  • Humans
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic / physiology
  • RNA, Messenger / metabolism
  • Retina / embryology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Species Specificity
  • Trans-Activators / genetics*
  • Transcription, Genetic*


  • 3' Untranslated Regions
  • 5' Untranslated Regions
  • Homeodomain Proteins
  • RNA, Messenger
  • Trans-Activators
  • cone rod homeobox protein

Associated data

  • GENBANK/AF335246
  • GENBANK/AF335248
  • GENBANK/AF335593
  • GENBANK/AF335594
  • GENBANK/AF335595
  • GENBANK/AF335596