Detection of oxazolidinone-resistant Enterococcus faecalis and Enterococcus faecium strains by real-time PCR and PCR-restriction fragment length polymorphism analysis

J Clin Microbiol. 2002 Nov;40(11):4298-300. doi: 10.1128/JCM.40.11.4298-4300.2002.

Abstract

A real-time PCR assay identified linezolid-resistant Enterococcus faecalis and Enterococcus faecium isolates with a G2576U rRNA mutation. PCR-restriction fragment length polymorphism analysis of ribosomal DNA amplicons with NheI also detected this mutation. Both assays detected isolates heterozygous at this position. Recognition of isolates with what is presently the most frequent oxazolidinone resistance mutation may aid surveillance and individual case management.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • DNA, Ribosomal / analysis
  • Deoxyribonucleases, Type II Site-Specific / genetics
  • Drug Resistance, Bacterial / genetics*
  • Enterococcus faecalis / drug effects*
  • Enterococcus faecalis / genetics
  • Enterococcus faecium / drug effects*
  • Enterococcus faecium / genetics
  • Humans
  • Microbial Sensitivity Tests
  • Mutation
  • Oxazolidinones / pharmacology*
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • RNA, Ribosomal / genetics

Substances

  • Anti-Bacterial Agents
  • DNA, Ribosomal
  • Oxazolidinones
  • RNA, Ribosomal
  • endodeoxyribonuclease NheI
  • Deoxyribonucleases, Type II Site-Specific