A Cajal body-specific pseudouridylation guide RNA is composed of two box H/ACA snoRNA-like domains

Nucleic Acids Res. 2002 Nov 1;30(21):4643-9. doi: 10.1093/nar/gkf592.


Site-specific post-transcriptional conversion of uridines to pseudouridine in ribosomal RNAs and small nuclear RNAs (snRNAs) is directed by guide RNAs which possess the conserved box H and ACA sequence elements and fold into the consensus 'hairpin-hinge-hairpin-tail' secondary structure. Here, we describe an unusual mammalian pseudouridylation guide RNA, called U93, that is composed of two tandemly arranged box H/ACA RNA domains. The U93 RNA therefore carries two H and two ACA box motifs, all of which are essential for accumulation of the full-length RNA. The human U93 RNA accumulates in Cajal (coiled) bodies and it is predicted to function in pseudouridylation of the U2 spliceosomal snRNA. Our results lend further support to the notion that modification of the RNA polymerase II-transcribed spliceosomal snRNAs takes place in Cajal bodies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • COS Cells
  • Carps / genetics
  • Cattle
  • Coiled Bodies / genetics*
  • Coiled Bodies / metabolism
  • HeLa Cells
  • Humans
  • In Situ Hybridization
  • Mice
  • Molecular Sequence Data
  • Nucleic Acid Conformation*
  • Pseudouridine / metabolism*
  • RNA, Guide, Kinetoplastida / chemistry*
  • RNA, Guide, Kinetoplastida / genetics
  • RNA, Guide, Kinetoplastida / metabolism*
  • RNA, Small Nuclear / genetics
  • RNA, Small Nuclear / metabolism
  • RNA, Small Nucleolar / chemistry*
  • RNA, Small Nucleolar / genetics
  • Ribonucleoproteins, Small Nucleolar / genetics
  • Ribonucleoproteins, Small Nucleolar / metabolism
  • Spliceosomes / metabolism


  • GAR1 protein, human
  • GAR1 protein, mammalian
  • RNA, Guide
  • RNA, Small Nuclear
  • RNA, Small Nucleolar
  • Ribonucleoproteins, Small Nucleolar
  • U2 small nuclear RNA
  • Pseudouridine

Associated data

  • GENBANK/AF492209