Endothelial nitric oxide synthase gene and the development of diabetic nephropathy

Diabetes Res Clin Pract. 2002 Dec;58(3):179-85. doi: 10.1016/s0168-8227(02)00156-0.


Background: Endothelial nitric oxide synthase gene and the development of diabetic nephropathy

Background: Intron 4 insertion/deletion polymorphism of the constitutive endothelial nitric oxide synthase (ecNOS) gene may be related to diabetic nephropathy.

Methods: A case-control study was performed in three groups of Japanese patients with Type 2 diabetes mellitus, which including 123 patients with advanced diabetic nephropathy, 107 patients with overt proteinuria and normal serum creatinine level, and a control group of 203 patients with normal renal function despite having diabetes for over 10 years. Additionally, logistic regression analysis was used to assess the findings.

Results: When we examined the a-deletion/b-insertion in intron 4 of ecNOS gene, the genotype and allele frequencies were not significantly different between the patients with advanced diabetic nephropathy (a/a 2.4, a/b 21.9, b/b 75.5, 'a' 13.4, 'b' 86.6%), the patients with overt proteinuria (a/a 2.8, a/b 15.8, b/b 81.4, 'a' 10.7, 'b' 89.3%) and the control group (a/a 1.4, a/b 21.6, b/b 76.8, 'a' 12.8, 'b' 87.7%). Logistic regression analysis showed that the ecNOS intron4 a-allele frequency was not the related to nephropathy (P = 0.88).

Conclusion: We conclude that there is no association of the ecNOS gene polymorphism with the development of diabetic nephropathy in Japanese patients with type 2 diabetes.

Publication types

  • Comparative Study

MeSH terms

  • Albuminuria / genetics
  • Base Sequence
  • Case-Control Studies
  • DNA Primers
  • Diabetes Mellitus, Type 2 / genetics*
  • Diabetic Nephropathies / enzymology
  • Diabetic Nephropathies / epidemiology*
  • Diabetic Nephropathies / genetics*
  • Female
  • Genotype
  • Humans
  • Introns
  • Japan / epidemiology
  • Male
  • Mutagenesis, Insertional
  • Nitric Oxide Synthase / genetics*
  • Nitric Oxide Synthase Type III
  • Polymorphism, Genetic*
  • Reference Values
  • Regression Analysis
  • Risk Factors
  • Sequence Deletion


  • DNA Primers
  • NOS3 protein, human
  • Nitric Oxide Synthase
  • Nitric Oxide Synthase Type III