In vitro detection of apoptosis in monocytes/macrophages infected with human coronavirus

Clin Diagn Lab Immunol. 2002 Nov;9(6):1392-5. doi: 10.1128/cdli.9.6.1392-1395.2002.


Human coronavirus (HCoV) strain 229E infection, but not HCoV strain OC43 infection, of monocytes/macrophages from healthy donors and patients with multiple sclerosis in remission resulted in increased apoptosis, as measured by DNA changes and annexin V staining. Apoptosis correlated with the differential release of infectious virus. HCoV strain 229E titers were 10(3.5) to 10(6) 50% tissue culture-infective doses (TCID(50))/ml, and HCoV strain OC43 titers were only 10(1.2) to 10(2.7) TCID(50)/ml.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Viral / analysis
  • Apoptosis*
  • Cells, Cultured
  • Coronavirus / pathogenicity*
  • Cytokines / biosynthesis
  • Flow Cytometry
  • Humans
  • Macrophages / pathology
  • Macrophages / virology*
  • Monocytes / pathology
  • Monocytes / virology*
  • Multiple Sclerosis / virology


  • Antigens, Viral
  • Cytokines