Objective: To establish a new extrahepatic growing hepatocellular carcinoma cell line.
Methods: A specimen from extrahepatic growing hepatocellular carcinoma was cultured in vitro. Cancer cells were studied morphologically and subjected to karyotype analysis, DNA analysis, and tumor formation evaluation.
Results: Morphological observation and functional analysis showed that their features were similar to those of HCC. Chromosomes with a variation of 76 approximately 104 were able to secret AFP in vitro and to form bile canaliculi with microvilli.
Conclusion: EGHC-9901 cell line has characteristics of the extrahepatic growing hepatocellular carcinoma.