Negative autoregulation speeds the response times of transcription networks

J Mol Biol. 2002 Nov 8;323(5):785-93. doi: 10.1016/s0022-2836(02)00994-4.

Abstract

Cells regulate gene expression using networks of transcription interactions; it is of interest to discover the principles that govern the dynamical behavior of such networks. An important characteristic of these systems is the rise-time: the delay from the initiation of production until half maximal product concentration is reached. Here we employ synthetic gene circuits in Escherichia coli to measure the rise-times of non-self-regulated and of negatively autoregulated transcription units. Non-self-regulated units have a rise-time of one cell-cycle. We demonstrate experimentally that negative autoregulation feedback (also termed autogenous control) reduces the rise-time to about one fifth of a cell-cycle. This agrees with an analytical solution of a mathematical model for negative autoregulation. This may help in understanding the function of negative autoregulation, which appears in over 40% of known transcription factors in E.coli.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / metabolism
  • Feedback, Physiological
  • Gene Expression Regulation
  • Genes, Bacterial
  • Homeostasis*
  • Kinetics
  • Models, Biological
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transcription, Genetic*

Substances

  • Escherichia coli Proteins
  • Transcription Factors