Attenuation of Fungal Infection in Thermoregulating Locusta Migratoria Is Accompanied by Changes in Hemolymph Proteins

J Invertebr Pathol. 2002 Sep;81(1):19-24. doi: 10.1016/s0022-2011(02)00117-9.

Abstract

Hemolymph proteins in the locust, Locusta migratoria migratorioides infected with the fungus Metarhizium anisopliae var acridum were analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Under conditions that allowed locusts to thermoregulate, 2 proteins, ITB1 (ca. 18kDa) and ITB2 (ca. 13kDa) were induced 48h post inoculation. In contrast, under non-thermoregulating conditions, only 1 band, INTB1 (ca. 18kDa) was induced with similar molecular mass to ITB1. ITB1 and ITB2 were N-terminally sequenced but showed little homology to known proteins. The induction of hemolymphal proteins in infected, thermoregulating locusts and implication in insect immune defence are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Temperature Regulation*
  • Grasshoppers / microbiology*
  • Grasshoppers / physiology*
  • Hemolymph / metabolism*
  • Mycoses / physiopathology*
  • Proteins / metabolism

Substances

  • Proteins