Characterization of a novel human UDP-GalNAc transferase, pp-GalNAc-T10

FEBS Lett. 2002 Nov 6;531(2):115-21. doi: 10.1016/s0014-5793(02)03399-9.

Abstract

A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (pp-GalNAc-T) gene family was cloned as a homolog of human pp-GalNAc-T7, and designated pp-GalNAc-T10. pp-GalNAc-T10 transcript was found in the small intestine, stomach, pancreas, ovary, thyroid gland and spleen. In a polypeptide GalNAc-transfer assay, recombinant pp-GalNAc-T10 transferred GalNAc onto a panel of mucin-derived peptide substrates. Furthermore, pp-GalNAc-T10 demonstrated strong transferase activity with glycopeptide substrates.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chromatography, High Pressure Liquid
  • Cloning, Molecular
  • Glycosylation
  • Humans
  • Molecular Sequence Data
  • N-Acetylgalactosaminyltransferases / genetics*
  • N-Acetylgalactosaminyltransferases / metabolism*
  • Peptides / metabolism
  • Phylogeny
  • RNA, Messenger / biosynthesis
  • Substrate Specificity
  • Tissue Distribution

Substances

  • Peptides
  • RNA, Messenger
  • N-Acetylgalactosaminyltransferases
  • polypeptide N-acetylgalactosaminyltransferase

Associated data

  • GENBANK/AB078145