Altered shoot/root Na+ distribution and bifurcating salt sensitivity in Arabidopsis by genetic disruption of the Na+ transporter AtHKT1

FEBS Lett. 2002 Nov 6;531(2):157-61. doi: 10.1016/s0014-5793(02)03488-9.

Abstract

Sodium (Na+) is toxic to most plants, but the molecular mechanisms of plant Na+ uptake and distribution remain largely unknown. Here we analyze Arabidopsis lines disrupted in the Na+ transporter AtHKT1. AtHKT1 is expressed in the root stele and leaf vasculature. athkt1 null plants exhibit lower root Na+ levels and are more salt resistant than wild-type in short-term root growth assays. In shoot tissues, however, athkt1 disruption produces higher Na+ levels, and athkt1 and athkt1/sos3 shoots are Na+-hypersensitive in long-term growth assays. Thus wild-type AtHKT1 controls root/shoot Na+ distribution and counteracts salt stress in leaves by reducing leaf Na+ accumulation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Arabidopsis / drug effects
  • Arabidopsis / genetics
  • Arabidopsis / growth & development*
  • Arabidopsis Proteins / genetics*
  • Arabidopsis Proteins / physiology*
  • Cation Transport Proteins / genetics*
  • Cation Transport Proteins / physiology*
  • Culture Techniques
  • Mutation
  • Plant Leaves / anatomy & histology
  • Plant Leaves / drug effects
  • Plant Leaves / metabolism
  • Plant Proteins*
  • Plant Roots / anatomy & histology
  • Plant Roots / chemistry
  • Plant Roots / growth & development
  • Plant Shoots / anatomy & histology
  • Plant Shoots / chemistry
  • Plant Shoots / growth & development
  • Plants, Genetically Modified
  • RNA, Plant / biosynthesis
  • Seedlings / anatomy & histology
  • Seedlings / drug effects
  • Seedlings / growth & development
  • Sodium / analysis*
  • Sodium Chloride / pharmacology*
  • Symporters*

Substances

  • Arabidopsis Proteins
  • Cation Transport Proteins
  • HKT1 protein, Arabidopsis
  • HKT1 protein, plant
  • Plant Proteins
  • RNA, Plant
  • Symporters
  • Sodium Chloride
  • Sodium