B lymphocyte memory: role of stromal cell complement and FcgammaRIIB receptors

J Exp Med. 2002 Nov 4;196(9):1189-99. doi: 10.1084/jem.20021110.


To dissect the influence of CD21/CD35 and FcgammaRIIB in antigen retention and humoral memory, we used an adoptive transfer model in which antigen-primed B and T lymphocytes were given to sublethally irradiated wild-type mice or mice deficient in CD21/CD35 (Cr2(-/-)) or FcgammaRIIB receptors (FcgammaRIIB(-/-)). Cr2(-/-) chimeras showed impaired memory as characterized by a decrease in antibody titer, reduced frequency of antibody secreting cells, an absence of affinity maturation, and significantly reduced recall response. The impaired memory in Cr2(-/-) chimeras corresponded with the reduced frequency of antigen-specific memory B cells. Interestingly, FcgammaRIIB(-/-) chimeras showed a differential phenotype with impaired splenic but normal bone marrow responses. These data suggest that CD21/CD35 on stroma, including follicular dendritic cells, is critical to the maintenance of long-term B lymphocyte memory.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies / blood
  • Antigens, CD / immunology*
  • B-Lymphocytes / immunology*
  • Haptens
  • Hemocyanins / immunology
  • Immunologic Memory / immunology*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Receptors, Complement 3b / immunology*
  • Receptors, Complement 3d / immunology*
  • Receptors, IgG / immunology*
  • Stromal Cells / immunology*
  • Time Factors


  • 4-hydroxy-3-nitrophenylacetyl-keyhole limpet hemocyanin
  • Antibodies
  • Antigens, CD
  • Fc gamma receptor IIB
  • Haptens
  • Receptors, Complement 3b
  • Receptors, Complement 3d
  • Receptors, IgG
  • Hemocyanins