Penetration of topically administered ofloxacin and trimethoprim into aqueous humor

J Ocul Pharmacol Ther. 2002 Oct;18(5):445-53. doi: 10.1089/10807680260362722.


Ocular penetration of two topical antibiotics used to treat bacterial conjunctivitis was assessed in adult volunteers scheduled for cataract surgery. In this randomized, parallel-group study, patients instilled trimethoprim sulfate 0.1%/polymyxin B (n = 23) or ofloxacin 0.3% (n = 25) QID for 3 days, plus 4 instillations in the hour before surgery. Analysis of aqueous humor samples obtained during surgery showed a 2.4-fold greater concentration of ofloxacin over trimethoprim (1.135 micro g/ml vs 0.470 micro g/ml; P <.0001). The greater concentration of ofloxacin in ocular tissue coupled with its superior antibacterial activity profile supports its use as an alternative to trimethoprim/polymyxin B for treatment of bacterial conjunctivitis.

Publication types

  • Clinical Trial
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Anti-Infective Agents, Local / pharmacokinetics*
  • Aqueous Humor / metabolism*
  • Cataract Extraction
  • Chromatography, High Pressure Liquid
  • Female
  • Humans
  • Instillation, Drug
  • Male
  • Mass Spectrometry
  • Middle Aged
  • Ofloxacin / pharmacokinetics*
  • Ophthalmic Solutions
  • Permeability
  • Trimethoprim / pharmacokinetics*


  • Anti-Infective Agents, Local
  • Ophthalmic Solutions
  • Ofloxacin
  • Trimethoprim