Inhibitory effect of stevioside on tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in two-stage carcinogenesis in mouse skin

Biol Pharm Bull. 2002 Nov;25(11):1488-90. doi: 10.1248/bpb.25.1488.

Abstract

Four steviol (ent-kaurene-type diterpenoid) glycosides, stevioside, rebaudiosides A and C, and dulcoside A, have been isolated from Stevia rebaudiana BERTONI. These compounds showed strong inhibitory activity against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in mice. The 50% inhibitory dose of these compounds for TPA-induced inflammation was 54.1-291.6 micro g/ear. Furthermore, at 1.0 and 0.1 mg/mouse of stevioside mixture, the mixture of these compounds markedly inhibited the promoting effect of TPA (1 micro g/mouse) on skin tumor formation initiated with 7,12-dimethylbenz[a]anthracene (50 micro g/mouse).

MeSH terms

  • Animals
  • Antineoplastic Agents, Phytogenic / chemistry
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Antineoplastic Agents, Phytogenic / therapeutic use
  • Diterpenes / chemistry
  • Diterpenes / pharmacology*
  • Diterpenes / therapeutic use
  • Diterpenes, Kaurane*
  • Drug Screening Assays, Antitumor / methods*
  • Female
  • Glucosides / chemistry
  • Glucosides / pharmacology*
  • Glucosides / therapeutic use
  • Mice
  • Mice, Inbred ICR
  • Skin Neoplasms / chemically induced
  • Skin Neoplasms / drug therapy*
  • Stevia / chemistry*
  • Tetradecanoylphorbol Acetate / antagonists & inhibitors*
  • Tetradecanoylphorbol Acetate / toxicity*

Substances

  • Antineoplastic Agents, Phytogenic
  • Diterpenes
  • Diterpenes, Kaurane
  • Glucosides
  • stevioside
  • Tetradecanoylphorbol Acetate