[Alkaline phosphatase isoenzymes in the serum of patients with renal insufficiency]

An Med Interna. 2002 Sep;19(9):449-52.
[Article in Spanish]


Objective: The aim of this study was to test the utility of serum alkaline phosphatase isoenzymes determination from patients with renal insufficiency.

Material and methods: Serum levels of alkaline phosphatase isoenzymes were determined in a group of 58 patients: 22 of them suffering acute renal insufficiency (ARI) and 36 with chronic renal failure (CRF) undergoing regular hemodialysis, results obtained were compared from a population of 30 healthy adults. Intestinal, bone, liver, macromolecular and intestinal variant isoenzymes, were separated by electrophoresis on agarose gel and quantified using a densitometer.

Results: Were found a significant increase the total alkaline phosphatase activity in both pathologic groups (p < 0.05 in ARI and p < 0.01 in CRF). Isoenzymatic profiles showed: increase of the bone fraction (p < 0.05 in ARI and p < 0.001 in CRF), decrease of the liver isoenzyme (p < 0.001) in both groups, macromolecular fraction elevated in acute patients (p < 0.05) and a significant increase of the intestinal and intestinal variant isoenzyme in the chronic patients (p < 0.001).

Conclusion: The renal insufficiency modified the normal distribution of alkaline phosphatase isoenzymes and the study of their serum levels could be an effective non-invasive marker, for the evaluation of bone disease and intestinal disorders associated with renal failure.

Publication types

  • English Abstract

MeSH terms

  • Adult
  • Aged
  • Alkaline Phosphatase / blood*
  • Bone Diseases / blood
  • Bone Diseases / etiology
  • Female
  • Gastrointestinal Diseases / blood
  • Gastrointestinal Diseases / etiology
  • Humans
  • Isoenzymes / blood
  • Male
  • Middle Aged
  • Renal Dialysis
  • Renal Insufficiency / blood*
  • Renal Insufficiency / complications
  • Renal Insufficiency / therapy


  • Isoenzymes
  • Alkaline Phosphatase