A multiplex GC-MS/MS technique for the sensitive and quantitative single-run analysis of acidic phytohormones and related compounds, and its application to Arabidopsis thaliana

Planta. 2002 Nov;216(1):44-56. doi: 10.1007/s00425-002-0866-6. Epub 2002 Nov 12.

Abstract

A highly sensitive and accurate multiplex gas chromatography-tandem mass spectrometry (GC-MS/MS) technique is reported for indole-3-acetic acid, abscisic acid, jasmonic acid, 12-oxo-phytodienoic acid and salicylic acid. The optimized setup allows the routine processing and analysis of up to 60 plant samples of between 20 and 200 mg of fresh weight per day. The protocol was designed and the equipment used was chosen to facilitate implementation of the method into other laboratories and to provide access to state-of-the-art analytical tools for the acidic phytohormones and related signalling molecules. Whole-plant organ-distribution maps for indole-3-acetic acid, abscisic acid, jasmonic acid, 12-oxo-phytodienoic acid and salicylic acid were generated for Arabidopsis thaliana (L.) Heynh. For leaves of A. thaliana, a spatial resolution of hormone quantitation down to approximately 2 mm(2) was achieved.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscisic Acid / analysis
  • Abscisic Acid / chemistry
  • Abscisic Acid / metabolism
  • Algorithms
  • Arabidopsis / chemistry*
  • Arabidopsis / metabolism
  • Biological Transport / physiology
  • Cyclopentanes / analysis
  • Cyclopentanes / chemistry
  • Cyclopentanes / metabolism
  • Fatty Acids, Unsaturated / analysis
  • Fatty Acids, Unsaturated / chemistry
  • Fatty Acids, Unsaturated / metabolism
  • Gas Chromatography-Mass Spectrometry / methods*
  • Indoleacetic Acids / analysis
  • Indoleacetic Acids / chemistry
  • Indoleacetic Acids / metabolism
  • Molecular Structure
  • Oxylipins
  • Plant Growth Regulators / analysis*
  • Plant Growth Regulators / chemistry
  • Plant Growth Regulators / metabolism
  • Salicylic Acid / analysis
  • Salicylic Acid / chemistry
  • Salicylic Acid / metabolism
  • Signal Transduction / physiology

Substances

  • Cyclopentanes
  • Fatty Acids, Unsaturated
  • Indoleacetic Acids
  • Oxylipins
  • Plant Growth Regulators
  • 12-oxophytodienoic acid
  • jasmonic acid
  • indoleacetic acid
  • Abscisic Acid
  • Salicylic Acid