The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway

Planta. 2002 Nov;216(1):187-92. doi: 10.1007/s00425-002-0906-2. Epub 2002 Nov 12.


The Arabidopsis thaliana (L.) Heynh. mutant delayed-dehiscence2-2 (dde2-2) was identified in an En1/Spm1 transposon-induced mutant population screened for plants showing defects in fertility. The dde2-2 mutant allele is defective in the anther dehiscence process and filament elongation and thus exhibits a male-sterile phenotype. The dde2-2 phenotype can be rescued by application of methyl jasmonate, indicating that the mutant is affected in jasmonic acid biosynthesis. The combination of genetic mapping and a candidate-gene approach identified a frameshift mutation in the ALLENE OXIDE SYNTHASE (AOS) gene, encoding one of the key enzymes of jasmonic acid biosynthesis. Expression analysis and genetic complementation of the dde2-2 phenotype by overexpression of the AOS coding sequence confirmed that the male-sterile phenotype is indeed caused by the mutation in the AOS gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetates / pharmacology
  • Amino Acid Sequence
  • Arabidopsis / drug effects
  • Arabidopsis / enzymology
  • Arabidopsis / genetics*
  • Chromosome Mapping
  • Chromosomes, Plant / genetics
  • Cyclopentanes / metabolism*
  • Cyclopentanes / pharmacology
  • Fertility / genetics
  • Genetic Complementation Test
  • Intramolecular Oxidoreductases / genetics*
  • Intramolecular Oxidoreductases / metabolism
  • Molecular Sequence Data
  • Mutation
  • Oxylipins
  • Sequence Homology, Amino Acid


  • Acetates
  • Cyclopentanes
  • Oxylipins
  • jasmonic acid
  • methyl jasmonate
  • Intramolecular Oxidoreductases
  • hydroperoxide isomerase