Abstract
bicoid (bcd) RNA localization requires the activity of exuperantia and swallow at sequential steps of oogenesis and is microtubule dependent. In a genetic screen, we identified two novel genes essential for bcd RNA localization. They encode maternal gamma-Tubulin37C (gammaTub37C) and gamma-tubulin ring complex protein 75 (Dgrip75), both of which are gamma-tubulin ring complex components. Mutations in these genes specifically affect bcd RNA localization, whereas other microtubule-dependent processes during oogenesis are not impaired. This provides direct evidence that a subset of microtubules organized by the gamma-tubulin ring complex is essential for localization of bcd RNA. At stage 10b, we find gammaTub37C and Dgrip75 anteriorly concentrated and propose the formation of a microtubule-organizing center at the anterior pole of the oocyte.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Centrosome / metabolism
-
Drosophila Proteins / genetics
-
Drosophila Proteins / metabolism*
-
Drosophila melanogaster / embryology
-
Drosophila melanogaster / genetics
-
Drosophila melanogaster / metabolism
-
Drosophila melanogaster / physiology
-
Female
-
Genes, Insect
-
Green Fluorescent Proteins
-
Homeodomain Proteins / genetics*
-
Luminescent Proteins / genetics
-
Luminescent Proteins / metabolism
-
Microtubule-Associated Proteins / genetics
-
Microtubule-Associated Proteins / metabolism*
-
Microtubule-Organizing Center / metabolism
-
Microtubules / metabolism
-
Mutagenesis
-
Oocytes / metabolism
-
Oogenesis / physiology*
-
RNA / metabolism*
-
RNA-Binding Proteins / genetics
-
RNA-Binding Proteins / metabolism
-
Recombinant Fusion Proteins / genetics
-
Recombinant Fusion Proteins / metabolism
-
Trans-Activators / genetics*
-
Tubulin / genetics
-
Tubulin / metabolism*
Substances
-
Drosophila Proteins
-
Grip75 protein, Drosophila
-
Homeodomain Proteins
-
Luminescent Proteins
-
Microtubule-Associated Proteins
-
RNA-Binding Proteins
-
Recombinant Fusion Proteins
-
Trans-Activators
-
Tubulin
-
bcd protein, Drosophila
-
gammaTub37C protein, Drosophila
-
swa protein, Drosophila
-
Green Fluorescent Proteins
-
RNA