Gamma-tubulin37C and Gamma-Tubulin Ring Complex Protein 75 Are Essential for Bicoid RNA Localization During Drosophila Oogenesis

Dev Cell. 2002 Nov;3(5):685-96. doi: 10.1016/s1534-5807(02)00301-5.

Abstract

bicoid (bcd) RNA localization requires the activity of exuperantia and swallow at sequential steps of oogenesis and is microtubule dependent. In a genetic screen, we identified two novel genes essential for bcd RNA localization. They encode maternal gamma-Tubulin37C (gammaTub37C) and gamma-tubulin ring complex protein 75 (Dgrip75), both of which are gamma-tubulin ring complex components. Mutations in these genes specifically affect bcd RNA localization, whereas other microtubule-dependent processes during oogenesis are not impaired. This provides direct evidence that a subset of microtubules organized by the gamma-tubulin ring complex is essential for localization of bcd RNA. At stage 10b, we find gammaTub37C and Dgrip75 anteriorly concentrated and propose the formation of a microtubule-organizing center at the anterior pole of the oocyte.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Centrosome / metabolism
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / metabolism
  • Drosophila melanogaster / physiology
  • Female
  • Genes, Insect
  • Green Fluorescent Proteins
  • Homeodomain Proteins / genetics*
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / metabolism*
  • Microtubule-Organizing Center / metabolism
  • Microtubules / metabolism
  • Mutagenesis
  • Oocytes / metabolism
  • Oogenesis / physiology*
  • RNA / metabolism*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Trans-Activators / genetics*
  • Tubulin / genetics
  • Tubulin / metabolism*

Substances

  • Drosophila Proteins
  • Grip75 protein, Drosophila
  • Homeodomain Proteins
  • Luminescent Proteins
  • Microtubule-Associated Proteins
  • RNA-Binding Proteins
  • Recombinant Fusion Proteins
  • Trans-Activators
  • Tubulin
  • bcd protein, Drosophila
  • gammaTub37C protein, Drosophila
  • swa protein, Drosophila
  • Green Fluorescent Proteins
  • RNA