Group II introns: highly specific endonucleases with modular structures and diverse catalytic functions

Methods. 2002 Nov;28(3):323-35. doi: 10.1016/s1046-2023(02)00239-6.


Group II introns are large catalytic RNAs with a remarkable repertoire of reactions. Here we present construct designs and protocols that were used to develop a set of kinetic frameworks for studying the structure and reaction mechanisms of group II introns and ribozymes derived from them. In addition, we discuss application of these systems to structure/function analysis of the ai5gamma group II intron.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Catalysis
  • Hydrolysis
  • Introns*
  • Kinetics
  • Nucleic Acid Conformation
  • RNA Splicing
  • RNA, Catalytic / chemistry*
  • RNA, Catalytic / physiology
  • Structure-Activity Relationship
  • Substrate Specificity


  • RNA, Catalytic