A neoantigenic determinant in the D-dimer fragment of fibrin

E V Lugovskoy; I N Kolesnikova; P G Gritsenko; E N Zolotareva; P Gaffney; W Nieuwenhuizen; S V Komisarenko

doi:10.1016/s0049-3848(02)00204-9

A neoantigenic determinant in the D-dimer fragment of fibrin

Thromb Res. 2002 Aug 15;107(3-4):151-6. doi: 10.1016/s0049-3848(02)00204-9.

Authors

E V Lugovskoy¹, I N Kolesnikova, P G Gritsenko, E N Zolotareva, P Gaffney, W Nieuwenhuizen, S V Komisarenko

Affiliation

¹ Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, 9 Leontovicha street, Kiev 01601, Ukraine.

PMID: 12431482
DOI: 10.1016/s0049-3848(02)00204-9

Abstract

Monoclonal antibody (mAb) III-3b binds D-dimer with K(d)=1.4 x 10(-10) M without cross-reaction with fibrin(ogen). The epitope for this mAb is in Bbeta134-190, presumably in Bbeta155-160. The latter site is buried in the coiled coil structure of fibrin(ogen) but it is exposed as a neoantigenic determinant in D-dimer upon plasmic lysis of fibrin. mAb III-3b may be used as a tool for immunodiagnostic quantification of D-dimer in blood plasma.

MeSH terms

Antibodies, Monoclonal / immunology
Antibody Affinity
Enzyme-Linked Immunosorbent Assay
Epitope Mapping
Epitopes*
Fibrin / immunology*
Fibrin Fibrinogen Degradation Products / immunology*
Humans
Immunoblotting

Substances

Antibodies, Monoclonal
Epitopes
Fibrin Fibrinogen Degradation Products
fibrin fragment D
Fibrin