DNA binding studies of ruthenium(II) complexes containing asymmetric tridentate ligands

J Inorg Biochem. 2002 Nov 25;92(3-4):165-70. doi: 10.1016/s0162-0134(02)00543-3.

Abstract

Absorption spectroscopy, fluorescence spectroscopy and viscosity measurements have been used to characterize the DNA binding of [Ru(tpy)(dppt)](2+) (tpy=2,2':6',2"-terpyridine, dppt=3-(1,10-phenanthrolin-2-yl)-5,6-diphenyl-as-triazine), [Ru(tpy)(pta)](2+) (pta=3-(1,10-phenanthrolin-2-yl)-as-triazino[5,6-f]acenaphthylene) and [Ru(tpy)(ptp)](2+) (ptp=3-(1,10-phenanthrolin-2-yl)-as-triazino[5,6-f]-phenanthrene). The results indicate that [Ru(tpy)(pta)](2+) and [Ru(tpy)(ptp)](2+) bind with CT-DNA in an intercalative mode, while [Ru(tpy)(dppt)](2+) binds with DNA by partial intercalation. The ligand planarity of the complex has a significant effect on DNA binding affinity increases in the order [Ru(tpy)(dppt)](2+)<[Ru(tpy)(pta)](2+)<[Ru(tpy)(ptp)](2+).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites
  • DNA / chemistry*
  • Fluorescence
  • Intercalating Agents / chemistry
  • Ligands
  • Ruthenium Compounds / chemistry*
  • Spectrum Analysis
  • Viscosity

Substances

  • Intercalating Agents
  • Ligands
  • Ruthenium Compounds
  • DNA
  • calf thymus DNA