A maximal effort trial in obese women carrying the beta2-adrenoceptor Gln27Glu polymorphism

J Physiol Biochem. 2002 Jun;58(2):103-8. doi: 10.1007/BF03179845.

Abstract

The aim of this study was to observe the relationship of the beta2-adrenoceptor (ADRB2) gene polymorphism Gln27Glu in a group of obese female subjects submitted to an acute physical activity test. Six Glu27/Glu27 obese women were compared with six Gln27/Gln27 obese women (coupled by age, BMI, waist circumference, percentage of fat mass and absence of smoking). These groups were selected after having genotyped 91 obese subjects (BMI>30 kg/m2, age 20-60). Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were used to genotype the obese population. The physical activity challenge consisted of a Maximal effort trial (VO2 max) following the Bruce protocol on a walking treadmill. The frequency for the Glu allele was 0.42, which is similar to other Caucasian populations. In basal conditions, subjects with the Glu27/Glu27 genotype showed a higher value of plasma insulin, while circulating glucose levels were similar in both groups. Also, diastolic arterial pressure was higher in the Glu27/Glu27 obese women. After the VO2 max trial, the Glu27 group had a significantly higher respiratory exchange ratio than the Gln27 group suggesting a lower post- exercise fat oxidation. These results provide evidence about a possible impact of the Gln27Glu polymorphism in the ADRB2 gene concerning a better response to exercise in obese Gln27 female subjects, in which basal insulinemia may play a role.

MeSH terms

  • Blood Glucose / analysis
  • Blood Pressure / genetics
  • Body Mass Index
  • Energy Metabolism
  • Exercise Test*
  • Female
  • Genotype
  • Glutamic Acid / genetics
  • Glutamine / genetics
  • Humans
  • Insulin / blood
  • Obesity / genetics*
  • Obesity / metabolism
  • Oxygen Consumption
  • Polymerase Chain Reaction / methods
  • Polymorphism, Genetic
  • Polymorphism, Restriction Fragment Length
  • Receptors, Adrenergic, beta-2 / genetics*
  • Statistics, Nonparametric

Substances

  • Blood Glucose
  • Insulin
  • Receptors, Adrenergic, beta-2
  • Glutamine
  • Glutamic Acid