Development of an immunofluorescence method for the detection of antibodies to Ebola virus subtype Reston by the use of recombinant nucleoprotein-expressing HeLa cells

Microbiol Immunol. 2002;46(9):633-8. doi: 10.1111/j.1348-0421.2002.tb02745.x.

Abstract

An indirect immunofluorescent assay (IFA) to detect Ebola virus subtype Reston (EBO-R) antibodies was developed by the use of a HeLa cell line stably expressing EBO-R nucleoprotein (NP). This IFA has a high specificity for the detection of EBO-R IgG antibodies in both hyperimmune rabbit sera and monkey sera collected during an EBO-R outbreak in the Philippines in 1996. Furthermore, this IFA showed a higher sensitivity for the detection of EBO-R antibodies than did the IFA using HeLa cells expressing the NP of Ebola virus subtype Zaire. These results suggest that this new IFA is useful for seroepidemiological studies of EBO-R infection among monkeys.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / analysis*
  • Ebolavirus / immunology*
  • Ebolavirus / isolation & purification
  • Fluorescent Antibody Technique, Indirect / methods*
  • HeLa Cells
  • Hemorrhagic Fever, Ebola / immunology
  • Humans
  • Macaca fascicularis
  • Monkey Diseases / virology
  • Nucleoproteins / biosynthesis
  • Nucleoproteins / genetics*
  • Nucleoproteins / metabolism
  • Plasmids
  • Rabbits
  • Recombinant Proteins / metabolism
  • Sensitivity and Specificity
  • Transfection / methods

Substances

  • Antibodies, Viral
  • Nucleoproteins
  • Recombinant Proteins