Ectopic expression of Tsi1 in transgenic hot pepper plants enhances host resistance to viral, bacterial, and oomycete pathogens

Mol Plant Microbe Interact. 2002 Oct;15(10):983-9. doi: 10.1094/MPMI.2002.15.10.983.

Abstract

In many plants, including hot pepper plants, productivity is greatly affected by pathogen attack. We reported previously that tobacco stress-induced gene 1 (Tsi1) may play an important role in regulating stress responsive genes and pathogenesis-related (PR) genes. In this study, we demonstrated that overexpression of Tsi1 gene in transgenic hot pepper plants induced constitutive expression of several PR genes in the absence of stress or pathogen treatment. The transgenic hot pepper plants expressing Tsi1 exhibited resistance to Pepper mild mottle virus (PMMV) and Cucumber mosaic virus (CMV). Furthermore, these transgenic plants showed increased resistance to a bacterial pathogen, Xanthomonas campestris pv. vesicatoria and also an oomycete pathogen, Phytophthora capsici. These results suggested that ectopic expression of Tsi1 in transgenic hot pepper plants enhanced the resistance of the plants to various pathogens, including viruses, bacteria, and oomycete. These results suggest that using transcriptional regulatory protein genes may contribute to developing broad-spectrum resistance in crop plants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / growth & development
  • Capsicum / genetics*
  • Capsicum / microbiology
  • Capsicum / virology
  • Cucumovirus / growth & development
  • DNA-Binding Proteins / genetics*
  • Gene Expression Regulation, Plant
  • Immunity, Innate / genetics
  • Oomycetes / growth & development
  • Phytophthora / growth & development
  • Plant Diseases / genetics*
  • Plant Diseases / microbiology
  • Plant Diseases / virology
  • Plant Proteins / genetics*
  • Plant Viruses / growth & development
  • Plants, Genetically Modified
  • Signal Transduction / genetics
  • Signal Transduction / physiology
  • Transcription Factors / genetics*
  • Xanthomonas campestris / growth & development

Substances

  • DNA-Binding Proteins
  • Plant Proteins
  • Transcription Factors
  • Tsi1 protein, Nicotiana tabacum
  • pathogenesis-related proteins, plant