Serum deprivation-induced upregulation of voltage-dependent sodium channels in adrenal chromaffin cells: selective involvement of extracellular signal-regulated kinase pathway

Toshihiko Yanagita; Hideyuki Kobayashi; Hiroki Yokoo; Tomokazu Saitoh; Shin-Ichi Minami; Seiji Shiraishi; Yasuhito Uezono; Akihiko Wada

doi:10.1111/j.1749-6632.2002.tb04453.x

Serum deprivation-induced upregulation of voltage-dependent sodium channels in adrenal chromaffin cells: selective involvement of extracellular signal-regulated kinase pathway

Ann N Y Acad Sci. 2002 Oct:971:153-5. doi: 10.1111/j.1749-6632.2002.tb04453.x.

Authors

Toshihiko Yanagita¹, Hideyuki Kobayashi, Hiroki Yokoo, Tomokazu Saitoh, Shin-Ichi Minami, Seiji Shiraishi, Yasuhito Uezono, Akihiko Wada

Affiliation

¹ Department of Pharmacology, Miyazaki Medical College, Miyazaki 889-1692, Japan. yanagita@post1.miyazaki-med.ac.jp

PMID: 12438109
DOI: 10.1111/j.1749-6632.2002.tb04453.x

Abstract

In the present study, we investigated whether activation of the MAPK family could regulate the cell surface expression of Na channels in cultured bovine adrenal chromaffin cells. The results suggest that constitutively activated ERK (but not p38 or JNK), by various extracellular stimuli, down-modulates the density of cell surface Na channels, which was mediated via the destabilization of Na channel alpha-subunit mRNA.

MeSH terms

Animals
Cattle
Chromaffin Cells / metabolism*
Culture Media, Serum-Free / pharmacology
Enzyme Activation
Immunoblotting
JNK Mitogen-Activated Protein Kinases
MAP Kinase Signaling System
Mitogen-Activated Protein Kinases / metabolism*
Phosphorylation
Protein Binding
RNA, Messenger / metabolism
Sodium Channels / metabolism*
Time Factors
Up-Regulation*
p38 Mitogen-Activated Protein Kinases

Substances

Culture Media, Serum-Free
RNA, Messenger
Sodium Channels
JNK Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinases
p38 Mitogen-Activated Protein Kinases