A new parvovirus genotype persistent in human skin

Virology. 2002 Oct 25;302(2):224-8. doi: 10.1006/viro.2002.1673.


Parvovirus B19 is the exclusive human pathogen of the Erythrovirus genus. In classical view, the B19 DNA sequence shows little variability, with no disease-specific or tissue type specific associations. We examined skin biopsies from patients with B19-unrelated skin disease or from constitutionally healthy adults by polymerase chain reaction assays for four different genomic regions of the B19 virus. Sequencing showed that the skin-derived viral DNA differed within the protein-coding region from the B19 reference sequences by 10.8% and from the V9 variant by 8.6% and within the noncoding region (covering nucleotides 189-435 of the promoter region) by 26.5 and 17.2%, respectively. Despite this sequence difference, the promoter region was shown by a luciferase gene expression assay to be biologically active. We have detected a new B19 virus genotype, K71, which differs extensively from the known B19-virus genotypes and is persistently carried in human skin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biopsy
  • Cell Line
  • DNA, Viral / analysis*
  • Genotype
  • Humans
  • Luciferases / metabolism
  • Molecular Sequence Data
  • Parvovirus B19, Human / classification*
  • Parvovirus B19, Human / genetics
  • Parvovirus B19, Human / isolation & purification*
  • Phylogeny
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Sequence Analysis, DNA
  • Skin / virology*
  • Transfection


  • DNA, Viral
  • Luciferases

Associated data

  • GENBANK/AY044266
  • GENBANK/AY044267
  • GENBANK/AY044268