Characterization of outer membrane efflux proteins OpmE, OpmD and OpmB of Pseudomonas aeruginosa: molecular cloning and development of specific antisera

FEMS Microbiol Lett. 2002 Nov 19;217(1):57-63. doi: 10.1111/j.1574-6968.2002.tb11456.x.

Abstract

The third genes, opmE, opmD and opmB, of multidrug efflux operons deduced from the Pseudomonas aeruginosa PAO1 genome data were cloned by polymerase chain reaction. The opmB gene product showed functional cooperation with inner membrane-associated components, MexAB, MexCD and MexXY, of the previously characterized multidrug efflux systems responsible for resistance to antimicrobial agents and extrusion of ethidium. The opmE and opmD gene products did not show functional cooperation. Immunoblots using a specific rabbit antiserum demonstrated, through exponential to stationary phases, constant expression of opmB and growth phase-dependent expression of opmD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Antibodies, Bacterial / immunology*
  • Antibody Specificity
  • Bacterial Outer Membrane Proteins / genetics*
  • Bacterial Outer Membrane Proteins / immunology*
  • Bacterial Outer Membrane Proteins / metabolism
  • Carrier Proteins / genetics*
  • Carrier Proteins / immunology*
  • Carrier Proteins / metabolism
  • Cells, Cultured
  • Cloning, Molecular
  • Drug Resistance, Multiple, Bacterial / genetics
  • Ethidium / metabolism
  • Microbial Sensitivity Tests
  • Mutation
  • Operon*
  • Pseudomonas aeruginosa / drug effects
  • Pseudomonas aeruginosa / genetics*
  • Pseudomonas aeruginosa / growth & development
  • Pseudomonas aeruginosa / immunology
  • Rabbits

Substances

  • Anti-Bacterial Agents
  • Antibodies, Bacterial
  • Bacterial Outer Membrane Proteins
  • Carrier Proteins
  • Ethidium