Peptide-beta2-microglobulin-MHC fusion molecules bind antigen-specific T cells and can be used for multivalent MHC-Ig complexes

J Immunol Methods. 2002 Dec 20;271(1-2):125-35. doi: 10.1016/s0022-1759(02)00346-0.


Recombinant soluble MHC molecules are widely used for visualization, activation and inhibition of antigen-specific immune responses. Using a genetic approach, we have generated two novel peptide-beta2-microglobulin-MHC constructs. We have linked the MHC molecule with the peptide of interest, without limiting the recognition by the cognate TCR. This molecule can also be joined with the IgG heavy chain resulting in a dimeric MHC-Ig fusion protein. These molecules bind antigen-specific T cells with high specificity and sensitivity, therefore, providing a valuable tool for detection as well as enrichment of antigen-specific T cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigen Presentation / immunology
  • COS Cells
  • Chlorocebus aethiops
  • DNA / chemistry
  • DNA / genetics
  • Epitopes, T-Lymphocyte / immunology*
  • Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
  • HLA-A2 Antigen / genetics
  • HLA-A2 Antigen / immunology*
  • Humans
  • Immunoglobulin G / genetics
  • Immunoglobulin G / immunology
  • Polymerase Chain Reaction
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology*
  • T-Lymphocytes / immunology
  • beta 2-Microglobulin / genetics
  • beta 2-Microglobulin / immunology*


  • Epitopes, T-Lymphocyte
  • HLA-A2 Antigen
  • Immunoglobulin G
  • Recombinant Fusion Proteins
  • beta 2-Microglobulin
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • DNA