The protein encoded at the 3' end of the serine protease gene of Aeromonas sobria functions as a chaperone in the production of the protease

Tomohiko Nomura; Yoshio Fujii; Hiroyasu Yamanaka; Hidetomo Kobayashi; Keinosuke Okamoto

doi:10.1128/JB.184.24.7058-7061.2002

The protein encoded at the 3' end of the serine protease gene of Aeromonas sobria functions as a chaperone in the production of the protease

J Bacteriol. 2002 Dec;184(24):7058-61. doi: 10.1128/JB.184.24.7058-7061.2002.

Authors

Tomohiko Nomura¹, Yoshio Fujii, Hiroyasu Yamanaka, Hidetomo Kobayashi, Keinosuke Okamoto

Affiliation

¹ Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro, Japan. nomu@ph.bunri-u.ac.jp

Abstract

For the successful production of Aeromonas sobria serine protease (ASP), open reading frame 2 (ORF2) protein, encoded at the 3' end of the protease operon, is required. In this study, we examined the action of ORF2 protein. The results showed that the protein associated with ASP in the periplasm and helped ASP to form an active structure.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aeromonas / enzymology*
Bacterial Proteins / physiology*
Molecular Chaperones / physiology*
Operon
Serine Endopeptidases / biosynthesis
Serine Endopeptidases / genetics*

Substances

Bacterial Proteins
Molecular Chaperones
Serine Endopeptidases