The WRP component of the WAVE-1 complex attenuates Rac-mediated signalling

Nat Cell Biol. 2002 Dec;4(12):970-5. doi: 10.1038/ncb886.


WAVE-1, which is also known as Scar, is a scaffolding protein that directs actin reorganization by relaying signals from the GTPase Rac to the Arp2/3 complex. Although the molecular details of WAVE activation by Rac have been described, the mechanisms by which these signals are terminated remain unknown. Here we have used tandem mass spectrometry to identify previously unknown components of the WAVE signalling network including WRP, a Rac-selective GTPase-activating protein. WRP binds directly to WAVE-1 through its Src homology domain 3 and specifically inhibits Rac function in vivo. Thus, we propose that WRP is a binding partner of WAVE-1 that functions as a signal termination factor for Rac.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Microfilament Proteins / physiology*
  • Molecular Sequence Data
  • Protein Binding
  • Rats
  • Rats, Sprague-Dawley
  • Sequence Alignment
  • Wiskott-Aldrich Syndrome Protein Family
  • rac GTP-Binding Proteins / physiology*


  • Microfilament Proteins
  • Wiskott-Aldrich Syndrome Protein Family
  • rac GTP-Binding Proteins

Associated data

  • GENBANK/AB007925
  • GENBANK/AF464189
  • GENBANK/AF496547
  • RefSeq/NM_001666
  • RefSeq/XM_051143