Planar polarity and actin dynamics in the epidermis of Drosophila

Nat Cell Biol. 2002 Dec;4(12):937-44. doi: 10.1038/ncb882.

Abstract

Dorsal closure is a morphogenetic process involving the coordinated convergence of two epithelial sheets to enclose the Drosophila melanogaster embryo. Specialized populations of cells at the edges of each epithelial sheet, the dorsal-most epidermal cells, emit actin-based processes that are essential for the proper enclosure of the embryo. Here we show that actin dynamics at the leading edge is preceded by a planar polarization of the dorsal-most epidermal cells associated with a reorganization of the cytoskeleton. An important consequence of this planar polarization is the formation of actin-nucleating centres at the leading edge, which are important in the dynamics of actin. We show that Wingless (Wg) signalling and Jun amino-terminal kinase (JNK) signalling have overlapping but different roles in these events.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / genetics
  • Actins / physiology*
  • Animals
  • Cell Polarity / genetics
  • Cell Polarity / physiology*
  • Drosophila Proteins / genetics
  • Drosophila Proteins / physiology
  • Drosophila melanogaster / cytology*
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / physiology*
  • Embryo, Nonmammalian / cytology
  • Embryo, Nonmammalian / physiology
  • Epidermal Cells*
  • Epidermis / physiology*
  • JNK Mitogen-Activated Protein Kinases*
  • MAP Kinase Kinase 4
  • Mitogen-Activated Protein Kinase Kinases / genetics
  • Mitogen-Activated Protein Kinase Kinases / physiology
  • Morphogenesis / genetics
  • Mutation
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / physiology
  • Signal Transduction / genetics
  • Signal Transduction / physiology*
  • Wnt1 Protein

Substances

  • Actins
  • Drosophila Proteins
  • Proto-Oncogene Proteins
  • Wnt1 Protein
  • wg protein, Drosophila
  • JNK Mitogen-Activated Protein Kinases
  • MAP Kinase Kinase 4
  • Mitogen-Activated Protein Kinase Kinases