Histopathology of natural Ebola virus subtype Reston infection in cynomolgus macaques during the Philippine outbreak in 1996

Exp Anim. 2002 Oct;51(5):447-55. doi: 10.1538/expanim.51.447.


We investigated the livers, spleens, kidneys and lungs collected from 24 cynomolgus macaques (Macaca fascicularis) naturally infected with Ebola virus subtype Reston (EBO-R) during the Philippine outbreak in 1996, in order to reveal the histopathologic findings. These macaques showed necrotic hepatocytes with inclusions, slight to massive fibrin deposition in splenic cords, depletion of lymphoid cells in the white pulp of the spleen, and fibrin thrombi in some organs. Immunohistochemical analysis using anti-leukocyte antigen L1 antibody revealed an increase in blood-derived macrophages/monocytes in the livers, kidneys and lungs of EBO-R infected macaques. EBO-R NP antigens were detected in the macrophages/monocytes, endothelial cells and fibroblasts in the liver, spleen, kidney and lung. These results indicate that EBO-R infection is characterized by systemic coagulopathy and an increase in blood-derived macrophages/monocytes in accordance with the EBO-R propagation in macrophages/monocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Laboratory
  • Disease Outbreaks
  • Ebolavirus / isolation & purification
  • Hemorrhagic Fever, Ebola / epidemiology
  • Hemorrhagic Fever, Ebola / pathology*
  • Hemorrhagic Fever, Ebola / veterinary*
  • Hemorrhagic Fever, Ebola / virology
  • Leukocyte L1 Antigen Complex
  • Macaca fascicularis*
  • Macrophages
  • Monkey Diseases / epidemiology
  • Monkey Diseases / pathology*
  • Monkey Diseases / virology
  • Monocytes
  • Philippines / epidemiology
  • Time Factors


  • Leukocyte L1 Antigen Complex