Involvement of CD27/CD70 interactions in antigen-specific cytotoxic T-lymphocyte (CTL) activity by perforin-mediated cytotoxicity

Clin Exp Immunol. 2002 Dec;130(3):424-30. doi: 10.1046/j.1365-2249.2002.02012.x.


CD27 molecules are shown to be essential in the regulation of the death, activation and differentiation of T and B cells. However, the influence of CD27 on cytotoxic T-cell function remains obscure. Autologous EBV transformed B-cell lines (LCL), which highly express CD27 ligand CD70, here stimulated T cells and induced the cytotoxic T-lymphocyte (CTL) activity via T-cell antigen receptors (TCR). The cytotoxicity against LCL was diminished when anti-CD70 blocking MoAb was added initially in the culture. Resting T cells killed more CD70-transfected P815 cells than wild type P815 cells in the presence of anti-CD3 MoAb as measured by a 4-h 51Cr release assay, and the cytotoxicity of both of the cell populations completely disappeared in the presence of concanamycin A (CMA). The expression of the perforin by the LCL-induced CTL in the presence of anti-CD70 blocking MoAb was diminished as compared with that without the blockage of CD27/CD70 interactions. The CTL induced by LCL did not kill Fas-transfected WR cells. CD27 signalling in the T cells did not affect Fas ligand (FasL) mRNA expression, LAK activity and IFN-gamma synthesis in humans. Our data demonstrate that CD27/CD70 interactions enhance the cytotoxicity of CTL in the induction phase through enhancement of killing activity induced via the perforin-dependent mechanism, but not via the Fas/FasL system.

MeSH terms

  • Antigens, CD*
  • B-Lymphocytes / immunology*
  • CD27 Ligand
  • Cell Line, Transformed
  • Cytotoxicity Tests, Immunologic
  • Epstein-Barr Virus Infections / immunology*
  • Fas Ligand Protein
  • Flow Cytometry
  • Humans
  • Killer Cells, Lymphokine-Activated / immunology
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / immunology
  • Membrane Proteins / immunology*
  • Perforin
  • Pore Forming Cytotoxic Proteins
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Lymphocytes, Cytotoxic / immunology*
  • Tumor Necrosis Factor Receptor Superfamily, Member 7 / immunology*


  • Antigens, CD
  • CD27 Ligand
  • CD70 protein, human
  • FASLG protein, human
  • Fas Ligand Protein
  • Membrane Glycoproteins
  • Membrane Proteins
  • Pore Forming Cytotoxic Proteins
  • Tumor Necrosis Factor Receptor Superfamily, Member 7
  • Perforin