ALL-1 Is a Histone Methyltransferase That Assembles a Supercomplex of Proteins Involved in Transcriptional Regulation

Mol Cell. 2002 Nov;10(5):1119-28. doi: 10.1016/s1097-2765(02)00740-2.

Abstract

ALL-1 is a member of the human trithorax/Polycomb gene family and is also involved in acute leukemia. ALL-1 is present within a stable, very large multiprotein supercomplex composed of > or =29 proteins. The majority of the latter are components of the human transcription complexes TFIID (including TBP), SWI/SNF, NuRD, hSNF2H, and Sin3A. Other components are involved in RNA processing or in histone methylation. The complex remodels, acetylates, deacetylates, and methylates nucleosomes and/or free histones. The complex's H3-K4 methylation activity is conferred by the ALL-1 SET domain. Chromatin immunoprecipitations show that ALL-1 and other complex components examined are bound at the promoter of an active ALL-1-dependent Hox a9 gene. In parallel, H3-K4 is methylated, and histones H3 and H4 are acetylated at this promoter.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blotting, Western
  • Cell Nucleus / metabolism
  • Chromatin / metabolism
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / metabolism*
  • HeLa Cells
  • Histone Methyltransferases
  • Histone-Lysine N-Methyltransferase*
  • Histones / metabolism
  • Homeodomain Proteins / metabolism
  • Humans
  • K562 Cells
  • Mass Spectrometry
  • Methylation
  • Methyltransferases / chemistry*
  • Methyltransferases / metabolism
  • Myeloid-Lymphoid Leukemia Protein
  • Precipitin Tests
  • Protein Binding
  • Protein Methyltransferases
  • Protein Processing, Post-Translational
  • Protein Structure, Tertiary
  • Proto-Oncogenes*
  • Silver Staining
  • Transcription Factors*
  • Transcription, Genetic*

Substances

  • Chromatin
  • DNA-Binding Proteins
  • Histones
  • Homeodomain Proteins
  • KMT2A protein, human
  • Transcription Factors
  • homeobox protein HOXA9
  • Myeloid-Lymphoid Leukemia Protein
  • Histone Methyltransferases
  • Methyltransferases
  • Protein Methyltransferases
  • Histone-Lysine N-Methyltransferase