Regulation of I-transposon activity in Drosophila: evidence for cosuppression of nonhomologous transgenes and possible role of ancestral I-related pericentromeric elements

Genetics. 2002 Nov;162(3):1197-209.

Abstract

We have previously shown that the activity of functional I retrotransposons (I factors) introduced into Drosophila devoid of such elements can be repressed by transgenes containing an internal fragment of the I factor itself and that this repressing effect presents the characteristic features of homology-dependent gene silencing or cosuppression. Here we show that the same transgenes can induce silencing of a nonhomologous reporter gene containing as the sole I-factor sequence its 100-bp promoter fragment. Silencing of the nonhomologous reporter gene shows strong similarities to I-factor cosuppression: It does not require any translation product from the regulating transgenes, sense and antisense constructs are equally potent, and the silencing effect is only maternally transmitted and fully reversible. A search for genomic I-like sequences containing domains with similarities to those of both the regulating and the reporter transgenes led to the identification of four such elements, which therefore could act as intermediates-or relays-in the cosuppression machinery. These results strongly suggest that ancestral transposition-defective I-related elements, which are naturally present in the Drosophila genome, may participate per se in the natural conditions of I-factor silencing.

MeSH terms

  • Animals
  • Base Sequence
  • Chloramphenicol O-Acetyltransferase / genetics
  • Chloramphenicol O-Acetyltransferase / metabolism
  • DNA Transposable Elements*
  • Drosophila / genetics*
  • Drosophila / physiology
  • Gene Expression Regulation / physiology*
  • Gene Silencing / physiology
  • Genes, Reporter / genetics
  • Genes, Reporter / physiology
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Transgenes*

Substances

  • DNA Transposable Elements
  • Chloramphenicol O-Acetyltransferase