Fundamentals of experimental design for cDNA microarrays

Nat Genet. 2002 Dec;32 Suppl:490-5. doi: 10.1038/ng1031.


Microarray technology is now widely available and is being applied to address increasingly complex scientific questions. Consequently, there is a greater demand for statistical assessment of the conclusions drawn from microarray experiments. This review discusses fundamental issues of how to design an experiment to ensure that the resulting data are amenable to statistical analysis. The discussion focuses on two-color spotted cDNA microarrays, but many of the same issues apply to single-color gene-expression assays as well.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • DNA, Complementary / analysis*
  • Gene Expression
  • Gene Expression Profiling / methods*
  • Mice
  • Models, Biological
  • Oligonucleotide Array Sequence Analysis / methods*
  • Reference Standards
  • Reproducibility of Results
  • Research Design
  • Statistics as Topic


  • DNA, Complementary