Post-analysis follow-up and validation of microarray experiments

Nat Genet. 2002 Dec;32 Suppl:509-14. doi: 10.1038/ng1034.

Abstract

Measurement of gene-expression profiles using microarray technology is becoming increasingly popular among the biomedical research community. Although there has been great progress in this field, investigators are still confronted with a difficult question after completing their experiments: how to validate the large data sets that are generated? This review summarizes current approaches to verifying global expression results, discusses the caveats that must be considered, and describes some methods that are being developed to address outstanding problems.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • DNA, Complementary / genetics
  • Forecasting
  • Gene Expression Profiling / methods
  • Gene Expression Profiling / standards*
  • Humans
  • Oligonucleotide Array Sequence Analysis / methods
  • Oligonucleotide Array Sequence Analysis / standards*
  • Proteins / genetics
  • Quality Control
  • RNA, Messenger / genetics
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • DNA, Complementary
  • Proteins
  • RNA, Messenger