Isolation and characterization of Xenopus Hey-1: a downstream mediator of Notch signaling

Dev Dyn. 2002 Dec;225(4):554-60. doi: 10.1002/dvdy.10192.

Abstract

Regulation of Notch signaling likely occurs, at least in part, at the level of basic helix-loop-helix (bHLH) transcription factors that function downstream of Suppressor of Hairless (Su(H)) in the Notch pathway. To begin to characterize modulation of Notch signaling during organogenesis, we examined the bHLH transcription factor, XHey-1 (hairy related-1) in early Xenopus laevis embryos. XHey-1 is expressed in numerous tissues during early development including the somites, head, embryonic kidneys, and heart. Importantly, the expression of XHey-1 was significantly altered in response to perturbation of Notch signaling by means of inducible constructs that served to either activate or suppress Notch signaling through Su(H) in a temporally controlled manner.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Basic Helix-Loop-Helix Transcription Factors
  • DNA-Binding Proteins / metabolism
  • Membrane Proteins / metabolism*
  • Protein Structure, Tertiary
  • Receptors, Notch
  • Repressor Proteins / chemistry*
  • Repressor Proteins / genetics*
  • Repressor Proteins / isolation & purification
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction*
  • Time Factors
  • Transcription Factors / metabolism
  • Transcription, Genetic
  • Xenopus laevis / genetics*
  • Xenopus laevis / metabolism*

Substances

  • Basic Helix-Loop-Helix Transcription Factors
  • DNA-Binding Proteins
  • Hairy, HRT1 protein
  • Membrane Proteins
  • Receptors, Notch
  • Repressor Proteins
  • Transcription Factors