Structural characterization of the mouse high growth deletion and discovery of a novel fusion transcript between suppressor of cytokine signaling-2 (Socs-2) and viral encoded semaphorin receptor (Plexin C1)

Gene. 2002 Oct 16;299(1-2):153-63. doi: 10.1016/s0378-1119(02)01052-1.

Abstract

The high growth (HG) mouse mutation is a 460 Kb deletion of chromosome 10 which causes a 30-50% increase in growth in the homozygous animal. We have shotgun sequenced six bacterial artificial chromosomes which span the length of the deletion to an average depth of 13.2x to generate a 649,868 bp sequence. Sequence analysis revealed the presence of three genes, suppressor of cytokine signaling-2 (Socs-2), caspase and RIP adaptor with death domain (Raidd/Cradd), and viral encoded semaphorin receptor (Plexin C1, viral encoded semaphorin receptor). The two deletion breakpoints lie in within the second introns of both Socs-2 and Plexin C1, resulting in the formation of a novel expressed fusion transcript between Socs-2 and Plexin C1 in HG mice. Expression of the fusion transcript, the presence of four splice variants of Raidd/Cradd and the exon structure of Socs-2 were illustrated using polymerase chain reaction. Genomic comparisons of the mouse and human sequence were used to verify the sequence assembly.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing*
  • Animals
  • Base Sequence
  • CRADD Signaling Adaptor Protein
  • Carrier Proteins / genetics
  • Cell Adhesion Molecules / genetics*
  • Chromosome Breakage / genetics
  • Chromosome Deletion*
  • DNA / chemistry
  • DNA / genetics
  • DNA-Binding Proteins*
  • Exons
  • Gene Expression
  • Genes / genetics
  • Humans
  • Introns
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Mutation
  • Nerve Tissue Proteins / genetics*
  • Proteins / genetics*
  • Repressor Proteins*
  • Sequence Analysis, DNA
  • Suppressor of Cytokine Signaling Proteins
  • Trans-Activators*

Substances

  • Adaptor Proteins, Signal Transducing
  • CRADD Signaling Adaptor Protein
  • CRADD protein, human
  • Carrier Proteins
  • Cell Adhesion Molecules
  • Cradd protein, mouse
  • DNA-Binding Proteins
  • Nerve Tissue Proteins
  • Proteins
  • Repressor Proteins
  • SOCS2 protein, human
  • Socs2 protein, mouse
  • Suppressor of Cytokine Signaling Proteins
  • Trans-Activators
  • plexin
  • DNA

Associated data

  • GENBANK/AF485344