Advances in lentiviral vector design for gene-modification of hematopoietic stem cells

Curr Opin Biotechnol. 2002 Oct;13(5):429-36. doi: 10.1016/s0958-1669(02)00346-4.

Abstract

Lentiviral vectors are more efficient at transducing quiescent hematopoietic stem cells than murine retroviral vectors. This characteristic is due to multiple karyophilic components of the lentiviral vector pre-integration complex. Lentiviral vectors are also able to carry more complex payloads than murine retroviral vectors, making it possible to deliver expression cassettes that direct either constitutive or targeted expression in various hematopoietic stem cell progeny.

Publication types

  • Review

MeSH terms

  • Animals
  • Cell Cycle / genetics
  • DNA, Recombinant / genetics
  • DNA, Recombinant / therapeutic use
  • Gene Expression Regulation, Viral*
  • Genetic Therapy / methods
  • Genetic Vectors / genetics*
  • Genetic Vectors / isolation & purification
  • Genetic Vectors / therapeutic use
  • Genome, Viral*
  • Hematopoietic Stem Cells / physiology*
  • Hematopoietic Stem Cells / virology
  • Humans
  • Lentivirus / genetics*
  • Mice
  • Recombination, Genetic
  • Safety
  • Transduction, Genetic / methods*

Substances

  • DNA, Recombinant