In vivo plasmid DNA electrotransfer

Curr Opin Biotechnol. 2002 Oct;13(5):443-7. doi: 10.1016/s0958-1669(02)00377-4.

Abstract

In vivo electrotransfer is a physical technique for gene delivery in various mammalian tissues, which involves the injection of plasmid DNA into a target tissue and administration of an electric field. Its ease of performance, as well as recent understanding of its mechanism and applications to different mammalian tissues such as skeletal muscle, liver, brain and tumors, makes it a powerful technique. It could be used in gene therapy and as a laboratory tool to study gene functions.

Publication types

  • Review

MeSH terms

  • Animals
  • DNA
  • Electromagnetic Fields
  • Electrophoresis / methods
  • Electroporation / methods*
  • Gene Expression Profiling / methods
  • Gene Expression Regulation / physiology*
  • Gene Transfer Techniques*
  • Genetic Therapy / methods*
  • Humans
  • Muscle, Skeletal / physiology
  • Neoplasms / therapy*
  • Plasmids / chemistry*
  • Plasmids / genetics
  • Plasmids / metabolism*

Substances

  • DNA