Short-term and long-term modulation of gene expression by antisense therapeutics

Curr Opin Biotechnol. 2002 Oct;13(5):468-72. doi: 10.1016/s0958-1669(02)00366-x.

Abstract

To achieve effective modulation of gene expression by antisense oligonucleotides, novel oligonucleotide chemistries that do not promote RNase H degradation of target RNA are needed. In addition to short-term oligonucleotide effects, long-term gene regulation can be accomplished by intracellularly expressed antisense RNAs delivered by viral vectors.

Publication types

  • Review

MeSH terms

  • Gene Expression Regulation / drug effects*
  • Genetic Therapy / methods
  • Oligonucleotides, Antisense / chemistry*
  • Oligonucleotides, Antisense / genetics*
  • Oligonucleotides, Antisense / pharmacology
  • Protein Biosynthesis*
  • RNA Processing, Post-Transcriptional / genetics
  • RNA, Antisense / chemistry*
  • RNA, Antisense / genetics*
  • RNA, Antisense / pharmacology
  • Ribonuclease H / chemistry
  • Ribonuclease H / genetics
  • Ribonuclease H / metabolism

Substances

  • Oligonucleotides, Antisense
  • RNA, Antisense
  • Ribonuclease H