Abstract
To achieve effective modulation of gene expression by antisense oligonucleotides, novel oligonucleotide chemistries that do not promote RNase H degradation of target RNA are needed. In addition to short-term oligonucleotide effects, long-term gene regulation can be accomplished by intracellularly expressed antisense RNAs delivered by viral vectors.
MeSH terms
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Gene Expression Regulation / drug effects*
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Genetic Therapy / methods
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Oligonucleotides, Antisense / chemistry*
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Oligonucleotides, Antisense / genetics*
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Oligonucleotides, Antisense / pharmacology
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Protein Biosynthesis*
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RNA Processing, Post-Transcriptional / genetics
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RNA, Antisense / chemistry*
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RNA, Antisense / genetics*
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RNA, Antisense / pharmacology
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Ribonuclease H / chemistry
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Ribonuclease H / genetics
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Ribonuclease H / metabolism
Substances
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Oligonucleotides, Antisense
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RNA, Antisense
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Ribonuclease H