Lymphotoxin-beta receptor mediates NEMO-independent NF-kappaB activation

FEBS Lett. 2002 Dec 4;532(1-2):45-51. doi: 10.1016/s0014-5793(02)03622-0.

Abstract

Lymphotoxin-beta receptor (LTbetaR) is a member of the tumor necrosis factor receptor (TNFR) superfamily that activates nuclear factor-kappaB (NF-kappaB) through the IkappaB kinase (IKK) complex, the core of which is comprised of IKK1, IKK2 and NF-kappaB essential modulator (NEMO). We demonstrate here that the LTbetaR signaling to NF-kappaB activation does not necessarily require NEMO, which is essential for TNFR signaling. In the absence of NEMO, the p50 and RelB, but not RelA subunits of NF-kappaB are found in the nuclear DNA binding complexes induced by the LTbetaR signaling. Our results thus disclose NEMO-independent NF-kappaB activation by LTbetaR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus
  • Animals
  • Cell Line
  • Cell Nucleus / metabolism
  • Cells, Cultured
  • I-kappa B Kinase
  • Lymphotoxin beta Receptor
  • Mice
  • Mice, Knockout
  • NF-kappa B / metabolism*
  • NF-kappa B p50 Subunit
  • Protein-Serine-Threonine Kinases / metabolism
  • Protein-Serine-Threonine Kinases / physiology*
  • Proteins / genetics
  • Proteins / physiology
  • Proto-Oncogene Proteins / metabolism
  • Receptors, Tumor Necrosis Factor / physiology*
  • Signal Transduction
  • TNF Receptor-Associated Factor 2
  • TNF Receptor-Associated Factor 5
  • Transcription Factor RelA
  • Transcription Factor RelB
  • Transcription Factors / metabolism
  • Transcriptional Activation

Substances

  • Ltbr protein, mouse
  • Lymphotoxin beta Receptor
  • NF-kappa B
  • NF-kappa B p50 Subunit
  • Proteins
  • Proto-Oncogene Proteins
  • Receptors, Tumor Necrosis Factor
  • Relb protein, mouse
  • TNF Receptor-Associated Factor 2
  • TNF Receptor-Associated Factor 5
  • Transcription Factor RelA
  • Transcription Factors
  • Transcription Factor RelB
  • Protein-Serine-Threonine Kinases
  • Chuk protein, mouse
  • I-kappa B Kinase
  • Ikbkb protein, mouse
  • Ikbke protein, mouse