Abstract
Lymphotoxin-beta receptor (LTbetaR) is a member of the tumor necrosis factor receptor (TNFR) superfamily that activates nuclear factor-kappaB (NF-kappaB) through the IkappaB kinase (IKK) complex, the core of which is comprised of IKK1, IKK2 and NF-kappaB essential modulator (NEMO). We demonstrate here that the LTbetaR signaling to NF-kappaB activation does not necessarily require NEMO, which is essential for TNFR signaling. In the absence of NEMO, the p50 and RelB, but not RelA subunits of NF-kappaB are found in the nuclear DNA binding complexes induced by the LTbetaR signaling. Our results thus disclose NEMO-independent NF-kappaB activation by LTbetaR.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Active Transport, Cell Nucleus
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Animals
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Cell Line
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Cell Nucleus / metabolism
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Cells, Cultured
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I-kappa B Kinase
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Lymphotoxin beta Receptor
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Mice
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Mice, Knockout
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NF-kappa B / metabolism*
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NF-kappa B p50 Subunit
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Protein Serine-Threonine Kinases / metabolism
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Protein Serine-Threonine Kinases / physiology*
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Proteins / genetics
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Proteins / physiology
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Proto-Oncogene Proteins / metabolism
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Receptors, Tumor Necrosis Factor / physiology*
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Signal Transduction
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TNF Receptor-Associated Factor 2
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TNF Receptor-Associated Factor 5
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Transcription Factor RelA
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Transcription Factor RelB
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Transcription Factors / metabolism
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Transcriptional Activation
Substances
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Ltbr protein, mouse
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Lymphotoxin beta Receptor
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NF-kappa B
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NF-kappa B p50 Subunit
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Proteins
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Proto-Oncogene Proteins
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Receptors, Tumor Necrosis Factor
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Relb protein, mouse
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TNF Receptor-Associated Factor 2
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TNF Receptor-Associated Factor 5
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Transcription Factor RelA
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Transcription Factors
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Transcription Factor RelB
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Protein Serine-Threonine Kinases
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Chuk protein, mouse
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I-kappa B Kinase
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Ikbkb protein, mouse
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Ikbke protein, mouse