Effect of a mouthrinse containing selected propolis on 3-day dental plaque accumulation and polysaccharide formation

Caries Res. 2002 Nov-Dec;36(6):445-8. doi: 10.1159/000066535.


The aim of this study was to evaluate the effect of a mouthrinse containing propolis SNB-RS on 3-day dental plaque accumulation. Six volunteers took part in a double-blind crossover study performed in two phases of 3 days. During each phase the volunteers refrained from all oral hygiene and rinsed with 20% sucrose solution 5 times a day to enhance dental plaque formation and with mouthrinse (placebo or experimental) twice a day. On the 4th day, the plaque index (PI) of the volunteers was scored and the supragingival dental plaque was analyzed for insoluble polysaccharide (IP). The PI (SD) for the experimental group was 0.78 (0.17), significantly less than for the placebo group, 1.41 (0.14). The experimental mouthrinse reduced the IP concentration in dental plaque by 61.7% compared to placebo (p < 0.05). An experimental mouthrinse containing propolis SNB-RS was thus efficient in reducing supragingival plaque formation and IP formation under conditions of high plaque accumulation.

Publication types

  • Clinical Trial
  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Anti-Infective Agents, Local / therapeutic use*
  • Cross-Over Studies
  • Dental Plaque / metabolism
  • Dental Plaque / prevention & control*
  • Double-Blind Method
  • Female
  • Glucosyltransferases / antagonists & inhibitors
  • Humans
  • Male
  • Mouthwashes / therapeutic use*
  • Polysaccharides / metabolism
  • Propolis / pharmacology
  • Propolis / therapeutic use*
  • Statistics, Nonparametric


  • Anti-Infective Agents, Local
  • Mouthwashes
  • Polysaccharides
  • Propolis
  • Glucosyltransferases